Version: ecceaec
Date: 2021-08-02
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Click on the buttons to display the fieldnames for the schemas.
| Field Name | description | example | used_for | category | regex | |
|---|---|---|---|---|---|---|
| 0 | SAMPLE_NAME_GPCF | Sample ID used within a single ILSe submission for GPCF. Limited to 20 characters. | 0001_hgla_w23T14_sk-3 | ILSe, ODCF | sample | [\w-]{1,20} |
| 1 | PROJECT | ODCF Project Name as registered in OTP | OE0538_DO0001 | GUIDE | sample | [\w-]{1,32} |
| 2 | PATIENT_ID | Individual ID for OTP | mmus_01234 | GUIDE, Odomlab | sample | [\w-]{1,32} |
| 3 | SPECIES | Scientific name of a species. Note that CAST/Ej is a strain of Mus musculus. | Mus musculus | Odomlab | sample | [\w-]{1,64} |
| 4 | STRAIN | Identifier for the strain used. Use EFO, if possible | CAST/Ej, C57BL/6, [CAST/EjxC57BL/6]F1 | Odomlab | sample | NaN |
| 5 | GENOME | Genome used for QC at GPCF | mm10 | ILSe | sample | NaN |
| 6 | INDIVIDUAL | ID for the individual (MOVI Number, Researcher's working ID) | None | Odomlab | sample | NaN |
| 7 | SAMPLE_TYPE | Sample Type used by OTP, describing samples *across* individual. (lowercase and dash(-) only, end with digit) | liver01, liver01-test01 | GUIDE, Odomlab | sample | [a-z0-9-]* |
| 8 | TISSUE | Descriptor of the tissue, if not given it's tried to parse from SAMPLE_TYPE. | liver, skin, brain | Odomlab | sample | NaN |
| 9 | GENOTYPE | Genotype according to EFO. Typically wildtype if not GMO. | wild type | Odomlab | sample | NaN |
| 10 | PHENOTYPE | Description of the phenotype | healthy, tumor | GUIDE, Odomlab | sample | NaN |
| 11 | SEX | Biological sex of the sampled individual. | female, male, unknown | ILSe, GUIDE, Odomlab | sample | NaN |
| 12 | DATE_OF_BIRTH | Birthday of individual (format YYYY-MM-DD) | 2020-03-31 | Odomlab | sample | [0-9]{4}-[0-9]{2}-[0-9]{2} |
| 13 | DATE_OF_DEATH | Date of death (format YYYY-MM-DD) | 2020-01-04 | Odomlab | sample | [0-9]{4}-[0-9]{2}-[0-9]{2} |
| 14 | WAY_OF_DEATH | How was the mouse/animal killed? | neck_fracture CO2_neck_fracture isofloran_neck_fracture | Odomlab | sample | NaN |
| 15 | TREATMENT | Any treatment / pertubation applied to the individual/ cell-line. | acetone 25w_DMBA/TPA 10w_DMBA/TPA DMBA/TPA_or_TPA TPA UV | Odomlab | sample | NaN |
| 16 | CELL_INPUT[TOTAL_ALIVE CELLS] | Number of cell, if available | 1234 | Odomlab | experiment | [0-9]* |
| 17 | TISSUE_PREP_METHOD | Brief description to method of tissue preparation | liver_ex Single cell MB RNA/DNATissueLyzerII Single cell C. Ernst 1. Enzymes 2. Trypsin 1. Trypsin 2. Enzymes skinbiopsy | Odomlab | experiment | NaN |
| 18 | NA_PREP_METHOD | Method (probably a kit) used to extract nucleic acid (NA) | Modified Qiagen DNeasy blood&tissue Qiagen Dneasy blood&tissue Qiagen RNeasy micro | Odomlab | experiment | NaN |
| 19 | NOTES | Any additional notes, comments for the submission | None | Odomlab | experiment | NaN |
| 20 | LIB_PREP_KIT | If using a new Kit, check first with ODCF because they need to register it to their database. Use their descriptor. | scRNA_10x_v3, WGS_NEBNext_ULTRA_II, Agilent SureSelect Pre-capture, Agilent SureSelect XT Low input Mouse AllExon, Agilent SureSelect Post-capture, NEBNext RNA Low Input - E6420 | GUIDE, Odomlab | experiment | NaN |
| 21 | AVERAGE_FRAGMENT_SIZE | Experimentally determined average fragment length (basepairs) if available | 500 | Odomlab | experiment | [0-9]* |
| 22 | BARCODETYPE | Description of barcode used | SureSelect 16 Rxn 10X Chromium v3 NEB 96 oligos SureSelect 96 rxn | Odomlab | experiment | NaN |
| 23 | BARCODE_WELL_I5 | Well position of the barcode | A7, N701, SI-GA-A6 | Odomlab | experiment | NaN |
| 24 | BARCODE_SEQ_I5 | Sequence of the I5 barcode | TTACCGAC, TATCCTCT | ILSe, Odomlab | experiment | [ATGC]* |
| 25 | BARCODE_WELL_I7 | Well position of the barcode | A7, S503 | Odomlab | experiment | NaN |
| 26 | BARCODE_SEQ_I7 | Sequence of the I7 barcode (for 10x-Single Cell, you need enter four rows to supply the barcodes and add a "_a,_b,_c, _d" suffix to the Sample Type) | TTACCGAC | ILSe, Odomlab | experiment | [ATGC]* |
| 27 | DNA_FRAGMENTATION_METHOD | Method used to fragment DNA | enzymatic sonicator | Odomlab | experiment | NaN |
| 28 | IMAGING_DATASET_ID | Imaging dataset for Spatial Transcriptomics | DOIMG-00001 | Odomlab | experiment | NaN |
| 29 | MULTIPLEX_NAME | Your initials followed by the date of submission format(ABYYYYMMDD) | MB20190923 | ILSe, Odomlab | sequencing | [A-Z]{2}[0-9]{4}[0-9]{2}[0-9]{2} |
| 30 | ILSE_NO | ILSE number as provided by GPCF/ILSe | 12345 | GUIDE, Odomlab | sequencing | [0-9]{5,} |
| 31 | SINGLE_CELL_WELL_LABEL | REQUIRED FOR SMARTSEQ2, combination of column and row | 1A | GUIDE | sequencing | [0-9]{1,2}[A-Z] |
| 32 | ANTIBODY_TARGET | Antibody target protein | HNF4A, CEBPA | Odomlab | other | NaN |
| 33 | ANTIBODY | Antibody name, manufacturer etc. | None | Odomlab | other | NaN |
| Field Name | description | example | used_for | category | regex | |
|---|---|---|---|---|---|---|
| 0 | SAMPLE_NAME_GPCF | Sample ID used within a single ILSe submission for GPCF. Limited to 20 characters. | 0001_hgla_w23T14_sk-3 | ILSe, ODCF | sample | [\w-]{1,20} |
| 1 | GENOME | Genome used for QC at GPCF | mm10 | ILSe | sample | NaN |
| 2 | SEX | Biological sex of the sampled individual. | female, male, unknown | ILSe, GUIDE, Odomlab | sample | NaN |
| 3 | BARCODE_SEQ_I5 | Sequence of the I5 barcode | TTACCGAC, TATCCTCT | ILSe, Odomlab | experiment | [ATGC]* |
| 4 | BARCODE_SEQ_I7 | Sequence of the I7 barcode (for 10x-Single Cell, you need enter four rows to supply the barcodes and add a "_a,_b,_c, _d" suffix to the Sample Type) | TTACCGAC | ILSe, Odomlab | experiment | [ATGC]* |
| 5 | MULTIPLEX_NAME | Your initials followed by the date of submission format(ABYYYYMMDD) | MB20190923 | ILSe, Odomlab | sequencing | [A-Z]{2}[0-9]{4}[0-9]{2}[0-9]{2} |
| 6 | PROJECT | ODCF Project Name as registered in OTP | OE0538_DO0001 | GUIDE | sample | [\w-]{1,32} |
| 7 | PATIENT_ID | Individual ID for OTP | mmus_01234 | GUIDE, Odomlab | sample | [\w-]{1,32} |
| 8 | SAMPLE_TYPE | Sample Type used by OTP, describing samples *across* individual. (lowercase and dash(-) only, end with digit) | liver01, liver01-test01 | GUIDE, Odomlab | sample | [a-z0-9-]* |
| 9 | PHENOTYPE | Description of the phenotype | healthy, tumor | GUIDE, Odomlab | sample | NaN |
| 10 | LIB_PREP_KIT | If using a new Kit, check first with ODCF because they need to register it to their database. Use their descriptor. | scRNA_10x_v3, WGS_NEBNext_ULTRA_II, Agilent SureSelect Pre-capture, Agilent SureSelect XT Low input Mouse AllExon, Agilent SureSelect Post-capture, NEBNext RNA Low Input - E6420 | GUIDE, Odomlab | experiment | NaN |
| 11 | ILSE_NO | ILSE number as provided by GPCF/ILSe | 12345 | GUIDE, Odomlab | sequencing | [0-9]{5,} |
| 12 | SINGLE_CELL_WELL_LABEL | REQUIRED FOR SMARTSEQ2, combination of column and row | 1A | GUIDE | sequencing | [0-9]{1,2}[A-Z] |
| 13 | SPECIES | Scientific name of a species. Note that CAST/Ej is a strain of Mus musculus. | Mus musculus | Odomlab | sample | [\w-]{1,64} |
| 14 | STRAIN | Identifier for the strain used. Use EFO, if possible | CAST/Ej, C57BL/6, [CAST/EjxC57BL/6]F1 | Odomlab | sample | NaN |
| 15 | INDIVIDUAL | ID for the individual (MOVI Number, Researcher's working ID) | None | Odomlab | sample | NaN |
| 16 | TISSUE | Descriptor of the tissue, if not given it's tried to parse from SAMPLE_TYPE. | liver, skin, brain | Odomlab | sample | NaN |
| 17 | GENOTYPE | Genotype according to EFO. Typically wildtype if not GMO. | wild type | Odomlab | sample | NaN |
| 18 | TREATMENT | Any treatment / pertubation applied to the individual/ cell-line. | acetone 25w_DMBA/TPA 10w_DMBA/TPA DMBA/TPA_or_TPA TPA UV | Odomlab | sample | NaN |
| 19 | WAY_OF_DEATH | How was the mouse/animal killed? | neck_fracture CO2_neck_fracture isofloran_neck_fracture | Odomlab | sample | NaN |
| 20 | DATE_OF_BIRTH | Birthday of individual (format YYYY-MM-DD) | 2020-03-31 | Odomlab | sample | [0-9]{4}-[0-9]{2}-[0-9]{2} |
| 21 | DATE_OF_DEATH | Date of death (format YYYY-MM-DD) | 2020-01-04 | Odomlab | sample | [0-9]{4}-[0-9]{2}-[0-9]{2} |
| 22 | TISSUE_PREP_METHOD | Brief description to method of tissue preparation | liver_ex Single cell MB RNA/DNATissueLyzerII Single cell C. Ernst 1. Enzymes 2. Trypsin 1. Trypsin 2. Enzymes skinbiopsy | Odomlab | experiment | NaN |
| 23 | CELL_INPUT[TOTAL_ALIVE CELLS] | Number of cell, if available | 1234 | Odomlab | experiment | [0-9]* |
| 24 | NA_PREP_METHOD | Method (probably a kit) used to extract nucleic acid (NA) | Modified Qiagen DNeasy blood&tissue Qiagen Dneasy blood&tissue Qiagen RNeasy micro | Odomlab | experiment | NaN |
| 25 | BARCODETYPE | Description of barcode used | SureSelect 16 Rxn 10X Chromium v3 NEB 96 oligos SureSelect 96 rxn | Odomlab | experiment | NaN |
| 26 | BARCODE_WELL_I5 | Well position of the barcode | A7, N701, SI-GA-A6 | Odomlab | experiment | NaN |
| 27 | BARCODE_WELL_I7 | Well position of the barcode | A7, S503 | Odomlab | experiment | NaN |
| 28 | DNA_FRAGMENTATION_METHOD | Method used to fragment DNA | enzymatic sonicator | Odomlab | experiment | NaN |
| 29 | AVERAGE_FRAGMENT_SIZE | Experimentally determined average fragment length (basepairs) if available | 500 | Odomlab | experiment | [0-9]* |
| 30 | IMAGING_DATASET_ID | Imaging dataset for Spatial Transcriptomics | DOIMG-00001 | Odomlab | experiment | NaN |
| 31 | ANTIBODY | Antibody name, manufacturer etc. | None | Odomlab | other | NaN |
| 32 | ANTIBODY_TARGET | Antibody target protein | HNF4A, CEBPA | Odomlab | other | NaN |
| 33 | NOTES | Any additional notes, comments for the submission | None | Odomlab | experiment | NaN |